Journal: Scientific Reports

Article Title: The citrus flavanone naringenin impairs dengue virus replication in human cells

doi: 10.1038/srep41864

Figure Lengend Snippet: Naringenin half maximal inhibitory concentration (IC 50 ) and selectivity index (SI) for anti-DENV activity in Huh7.5 cells.

Article Snippet: The human-derived Huh7.5 hepatoma cell line (ATCC PTA-8561) was grown in Dulbecco’s Modified Eagle Medium/nutrient mixture F-12 (D-MEM/F-12; Gibco, Grand Island, NY, USA) supplemented with 100 IU/μg/mL penicillin/streptomycin (Gibco, Grand Island, NY, USA) and 10% FBS at 37 °C in a humidified, 5% CO 2 controlled atmosphere.

Techniques: Concentration Assay, Activity Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: MK-571, a Cysteinyl Leukotriene Receptor 1 Antagonist, Inhibits Hepatitis C Virus Replication

doi: 10.1128/AAC.02078-19

Figure Lengend Snippet: In vitro cellular toxicities of the indicated compound in Huh7.5 cells

Article Snippet: Human hepatoma-derived Huh7.5 cells (Apath LLC) were cultured in complete Dulbecco’s modified Eagle medium (DMEM; ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum, 50 IU/ml penicillin, 100 μg/ml streptomycin, and 0.1 μg/ml amphotericin B (ThermoFisher Scientific).

Techniques: In Vitro

Journal: Scientific Reports

Article Title: Extract from Aphloia theiformis , an edible indigenous plant from Reunion Island, impairs Zika virus attachment to the host cell surface

doi: 10.1038/s41598-018-29183-2

Figure Lengend Snippet: A . theiformis extract exerts antiviral effect against 4 DENV clinical isolates and an epidemic Brazilian ZIKV strain. Four DENV serotypes (DENV-1, 2, 3 and 4) and a Brazilian epidemic strain of ZIKV (ZV BR2015/15261) were incubated with A . theiformis extract for 1 h at 37 °C. ( a ) Huh7.5 cells were left uninfected or were infected with DENV at an MOI of 0.1 or with ZIKV at an MOI of 0.4 for 48 h. Recombinant IFN-α 2A (200 IU.mL −1 ) was added 2 h post infection and used as positive control. The percentage of E positive cells was evaluated using the Operetta High-Content Imaging System (PerkinElmer). Results are means ± SD of three independent experiments. ( b ) The residual infectivity of treated particles was titrated in C6/36 cells using a foci-forming immunodetection assay. Data represent the means ± SD from three independent experiments. One-way ANOVA and Dunnett’s test (*p < 0.05; **p < 0.01; ***p < 0.001).

Article Snippet: Vero cells (ATCC, CCL-81) and human-derived Huh7.5 hepatoma cells (ATCC, PTA-8561TM; Manassas, Virginia, USA) were cultured in minimum essential medium (MEM: Gibco/Invitrogen, Carlsbad, CA) supplemented with 5% heat-inactivated foetal bovine serum (FBS), 2 mmol.L −1 L-Glutamine, 1 mmol.L −1 sodium pyruvate, 100 U.mL −1 of penicillin, 0.1 mg.mL −1 of streptomycin and 0.5 µg.mL −1 of fungizone (PAN Biotech) under a 5% CO 2 atmosphere at 37 °C.

Techniques: Incubation, Infection, Recombinant, Positive Control, Imaging, Immunodetection